Milk Thistle (Silybum marianum) Does not Neutralize the Toxicity of Sorrel (Rumex obtusifolius)

Abstract

alestinian ranges need an objective assessment of the toxicity of some plants that
are started to be tested in the first series of research work of its kind in Palestine. In
this paper, Sorrel (Rumex obtusifolius) is assayed for toxicity to the hepatic cell line
HepG2 besides an assessment of Milk Thistle (Silybum marianum) for possible
antidotal virtues using the cells viability test (MTT).
Up to a given concentration (31.25 µg of plant extract/ml of HepG2 cell culture
medium), Sorrel (Rumex obtusifolius) has growth stimulation virtues on HepG2 cell
line using the viability assay MTT. Afterwards (starting at 125 µg of plant extract/ml
of HepG2 cell culture medium), the viability lines decline in a directly proportional
manner as the concentration of the plant extract increases/ml of HepG2 medium.
When assayed for its effect on the viability of HepG2 cell line, Milk Thistle
(Silybum marianum) shows an unexpected low viability profile that seemingly
contradicts the classical antidotal uses of this plant. However, it is assayed for such
virtues in this paper but unsuccessfully at the concentrations employed tentatively.
These results can be used to adjust for finer concentrations of this plant to be used in
case of intoxication of animals and humans by Sorrel (Rumex obtusifolius) which is
consumed by both.